Thursday, January 22, 2015

DNA motion induced by electrokinetic flow near an Au coated nanopore surface as voltage controlled gate

We used fluorescence microscopy to investigate the diffusion and drift motion of λ DNA molecules on an Au-coated membrane surface near nanopores, prior to their translocation through solid-state nanopores. With the capability of controlling electric potential at the Au surface as a gate voltage, V gate , the motions of DNA molecules, which are presumably generated by electrokinetic flow, vary dramatically near the nanopores in our observations. We carefully investigate these DNA motions with different values of V gate in order to alter the densities and polarities of the counterions, which are expected to change the flow speed or direction, respectively. Depending on V gate , our observations have revealed the critical distance from a nanopore for DNA molecules to be attracted or repelled—DNA’s anisotropic and unsteady drifting motions and accumulations of DNA molecules near the nanopore entrance. Further finite element method (FEM) numerical si...

Manabu Sugimoto, Yuta Kato, Kentaro Ishida, Changbae Hyun, Jiali Li and Toshiyuki Mitsui

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