Friday, September 05, 2014

Generic phosphatase activity detection using zinc mediated aggregation modulation of polypeptide-modified gold nanoparticles




Nanoscale , 2014, Advance Article

DOI: 10.1039/C4NR02791D, Paper

Robert Selegard, Karin Enander, Daniel Aili

A generic plasmonic assay for rapid detection and characterization of a wide range of phosphatases using different natural substrates is described that exploit the enzymatic release of inorganic phosphate to control the aggregation of nanoparticles functionalized with a designed Zn2+ responsive polypeptide.

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